Abstract:AIM:To investigate avasculopathy-evaluating method using fundus fluorescein angiography(FFA)in mice with oxygen-induced retinopathy(OIR).
METHODS:OIR model was induced by exposure of mice from groups A and B(the retinopathy was known to be more severe in group B than that in group A by previous research)to high oxygen(75%)from postnatal day 7(P7)to P12(n=12 for each group)and returned to normal environment at P12. On P17, the mice from both groups were randomly assigned to accept FFA or high molecular weight fluorescein isothiocyanate dextran(FITC-Dextran)perfusion combined with stretched preparation of retina(each method involved 6 pups from each group). The retinal non-perfused areas were quantified and compared by using image analysis software.
RESULTS: FFA combined with image analysis software was able to quantify the retinal non-perfused areas, which was comparable to the results analyzed by FITC-Dextran perfusion combined with stretched preparation of retina. No statistical difference was found between the results obtained by the two methods(P>0.05).
CONCLUSION:FFA combined with image quantification analysis can be used in retinalvasculopathy evaluation in mouse OIR model.
