MicroRNA profiling on oxidative stress induced apoptosis of human lens epithelium

Abstract:

AIM: To identify the changes in microRNA(miRNA)profile of human lens epithelium(HLE)induced by H₂O₂ and the role of miRNA in oxidative stress induced apoptosis.

METHODS: HLE cell line HLE-B3 was treated by 100μmol/L H₂O₂ for 24h and the total RNA were isolated by Trizol reagent. miRNA profile was generated by miRCURY^TM LNA microRNA Array. The target genes of differentially expressed miRNAs were predicted by bioinformatics software.

RESULTS:Twenty-eight miRNAs showed significantly differential expression after H₂O₂ treatment, 18 miRNAs upregulated and 10 miRNAs downregulated. The differentially expressed miRNAs may involve in apoptosis of lens epithetium and development of cataract through targeting BCL2L2 and MIP.

CONCLUSION: H₂O₂ can induce dramatically changes in miRNA profile of HLE, which may play a pivotal role in the pathogenesis and development of cataract.