Intervention of tea polyphenols to NF-κB expression on H2O2-induced rat lens epithelial cells

Abstract:

AIM: To observe the influence of tea polyphenols(TP)to NF-κB expression on the H₂O₂-induced lens epithelial cells.

METHODS: SD rats transparent crystals were cultured in vitro by lens technology, and placed in a certain concentration of H₂O₂ in MEM medium. The experimental cataract lens model was established. Blank control group, H₂O₂ group and tea polyphenols(0.02mg/mL, 0.2mg/mL, 2mg/mL)treatment group were set and sampled at different time points(6, 12, 24, 48 hours). NF-κB mRNA expression was detected by RT-PCR, and NF-κB protein expression was detected by Western-blot in lens epithelial cells.

RESULTS: H₂O₂ raised the expression of NF-κB, and appeared time-dependent manner(P<0.01)in lens epithelial cells; different concentrations of tea polyphenols can inhibit the expression of NF-κB in lens epithelial cells, when TP concentration was 0.2mg/mL, the inhibitory effects was statistically significant(P<0.05).

CONCLUSION: TP can inhibit the expression of NF-κB in H₂O₂-induced lens epithelial cells, when TP concentration was 0.2mg/mL, the effect is statistically significant.