Abstract:AIM: To investigate the expression of Skp2,p27 and PTEN proteins in B-cell non—Hodgkin lymphoma(NHL) of ocular adnexa.METHODS: Paraffin sections were collected from patients suffering from B-cell NHL (n=30) and lymphadenosis (n=10) of ocular adnexa in Qingdao Affiliated Hospital from 1995 to 2011. Features (age, gender, pathogenetic locations, pathological type) can be used as factors to classify 30 cases of B-cell NHL. Lymphadenosis sections were selected to the control group. The expression of Skp2,p27 and PTEN proteins were detected by immunohistochemistry and the positive expression rate between lymphomas and lymphadenosis was compared. Spearman rank correlation was used to estimate the relationships among Skp2, p27 and PTEN in ocular lymphoma. RESULTS: The expression of three proteins were not related to age, gender, pathogenetic locations but related to pathologic type of tumors. In B-cell NHL specimens, the expression of p27kip1 and PTEN was lower than that in lymphadenosis (P<0.05), while the expression of Skp2 was higher.Skp2 labeling frequency was increased dramatically in plasmacytoma(PL) and diffuse large B-cell lymphoma(DLBCL) compared with mucosa-associated lymphoid tissue(MALT)(P<0.01). While p27kip1 and PTEN labeling frequency in PL and DLBCL was significantly lower than that of MALT(P<0.05,P<0.01). There was a negative correlation between p27kip1 and Skp2 in MALT(r=-0.134, x2=12.58,P＜0.05）while there was a positive correlation between p27kip1 and PTEN（ r=0.828, x2=20.66,P＜0.05）．The relationship between Skp2 and PTEN was also negative(r=-0.883, x2=20.52,P＜0.05).CONCLUSION: Over expression of Skp2 and lower expression of p27kip1 and PTEN play an important role in the tumorigenesis and different pathologic type in ocular adnexal tumors. The expression of three proteins correlates with each other in MALT. Combined examination of Skp2,p27 and PTEN is a valuable marker to predict the presence of the B-cell NHL of ocular adnexa and differentiate the pathologic type of tumors.
