Study on interference effect of siRNA for transforming growth factor β2 on human Tenon-s capsule fibroblasts

Abstract:

AIM: To investigate the effects of siRNA for transforming growth factor β2(TGF-β²) on the expression of TGF-β² mRNA in human Tenon-s capsule fibroblasts. METHODS: Human Tenon- s fibroblasts were transferred by TGF-β² siRNAs vector after separated, cultured and passaged three in vitro. RT-PCR was performed to evaluate the levels of TGF-β² mRNA at 24, 48 and 72 hours after transferred in transferred cells, and untransferred cells were as the controls. RESULTS: Separated human Tenon-s fibroblasts attached in 4 hours and confluent monolayer cells formed in 36 hours. The adherent cells displayed fibroblast-like or spindle shape by microscope observation. The expression of TGF-β2 mRNA of transferred group was reduced significantly in comparison with the control group，expression inhibition rates were 17.40%, 52.80% and 79.20% respectively in 24, 48 and 72 hours after transferred,and this interference function was strengthened with the time extending. CONCLUSION:The vector of siRNA specific for TGF-β² is of potent interference ability for TGF-β² mRNA expression in human Tenon-s capsule fibroblasts.