Protection of dendrobium nobile’s extractive on H2O2-induced lens epithelial cells damage

Abstract:

AIM:To investigate the protection of different polarity alkaloids (fat-soluble, water-soluble, low polar, weak polar) from dendrobium nobile on H2O2 induced damage of human lens epithelial cells (HLEC). METHODS:The H2O2 and HLEC were incubated with oxidative damage model . At the same time the different polarity alkaloid were added and sustained for 24 hours. Then detection of the proliferation of HLEC was with MTT and apoptosis was detected by FCM. RESULTS:The proliferation of LEC in H2O2 group was declined. The group of fat-soluble alkaloid and water-soluble alkaloid(25μg/L) were significantly increased compared to H2O2-induced group. The group of fat-soluble alkaloids could significantly reduce the H2O2-induced apoptosis of LEC(P<0.01). CONCLUSION: Fat-soluble alkaloids(12.5μg/L) extracted from dendrobium nobile can enhance the proliferation of LEC by anti-oxidation, and inhibit the apoptosis of LEC.