Abstract:AIM:To determine the expression of basic fibroblast growth factor(bFGF)in retinal pigment epithelial(RPE)cells from anterior and posterior part of guinea pig eyes with lens-induced myopia(LIM).
METHODS:Thirty two-week-aged guinea pigs were randomly divided into three groups, group A, group B and group C. Another five guinea pigs(10 eyes)were chosen as control group without any treatment. Concave lenses were worn by the 30 guinea pigs on either side of their eyes. After 6, 15 and 30d, the lenses were removed and optometry and axial length were used to make sure the myopia had formed. The retinal pigment epithelial cells of each group were cultured and passaged in vitro using enzymatic digestion method. The cultured cells at 3～6 generation was detect by immunocytolchemistory, Real-Time PCR and Western blotting to detect the expression of bFGF.
RESULTS:The expression of bFGF was located in the cytoplasm and nucleus. The results of immunocytolchemistory, Real-Time PCR and Western blotting showed that the expression of bFGF were detected at anterior and posterior part of eyes from experiment group and control group, the expression in experiment group at both part was significantly lower than those in control group(P<0.05). The positive percentage of expression of bFGF in experiment group decreased with the induced time(P<0.05). The expression of bFGF from anterior part of both experiment group and control group was as the same as that from posterior part of themselves(P>0.05).
CONCLUSION:The expression of bFGF from anterior and posterior part of experiment group was lower than those of control group.
