Inhibition effect of trichostatin A on human Tenon fibroblasts activation induced by transforming growth factor -β1

Abstract:

AIM:To investigate the effect of histone deacetylase inhibitor(HDACi)trichostatin A(TSA)on synthesis of collagenⅠand proliferation of human Tenon fibroblasts(HTFs)induced by transforming growth factor-β₁(TGF-β₁).

METHODS: Firstly, the effect of TSA at different concentrations(200, 400, 600 and 800nmol/L)on HTFs viability after 24h was detected using MTT proliferation assays. Then, the effect of TSA at 400nmol/L and 600nmol/L mixed with 5ng/mL TGF-β₁ on HTFs viability after 24h were investigated using MTT proliferation assays. Furthermore, the mRNA and protein expression of collagen Ⅰ in HTFs after treatment with TSA at different concentrations(400, 600nmol/L)mixed with 5ng/mL TGF-β₁ as well as 600nmol/L TSA were detected by RT-PCR and Western-blot.

RESULTS: Compared with control group, the results of MTT showed that HTFs viability decreased significantly after treated with TSA at 400, 600 and 800nmol/L(P<0.05). The HTFs proliferation induced by TGF-β₁ could be attenuated by TSA at 400 and 600nmol/L(P<0.05). The results of RT-PCR and Western-blot confirmed that TSA at 400 and 600nmol/L had reversal effect on up-regulated gene transcription and protein expression levels of collagen Ⅰ induced TGF-β₁.

CONCLUSION:TSA can inhibit the HTFs proliferation induced by TGF-β₁ and attenuate gene transcription and protein expression of collagen Ⅰ.