Effect of FTY720 inhibiting corneal neovascularition induced by sphingosine 1-phosphate

Abstract:

AIM: To explore the inhibiting effect of FTY720 on corneal neovascularization(CNV)of rat.

METHODS: MTT assay and cells scratch were adopted to observe hyperplasia of human umbilical vein endothelial cells(HUVECs)and cell migration induced by sphingosine-1-phosphate(S1P)after using FTY720 of different concentration. The effect of FTY720 on CNV induced by S1P in a rat corneal micropocket model was detected. 30SD rats were randomly divided into group A, group B and group C with 10 rats per group. S1P and 0μg, 5μg, and 20μg FTY720 controlled-released particles were implanted into the corneal stroma. The growth of CNV and having pathological examination on 12d after the operation was observed. Findings was analyzed by one-way ANOVA.

RESULTS: 10, 10², 10³, and 10⁴ nmol/L FTY720 and HUVECs co-incubate 72h could inhibit cell proliferation(P<0.01), 24h after the function of 10,100nmol/L FTY720, it could inhibit S1P-induced cell migration and the ability of restricting cell proliferation and cell migration was enhanced with increasing concentration of FTY720. On 12d, after rat corneal micropocket controlled-release particles was implanted into groups A, B, C, the CNV area were respectively 10.05±1.19, 6.59±0.95, 2.70±0.68mm²(F=145.155, P<0.01), group A and group B was statistically different and this was the same case between group B and group C(P<0.01).

CONCLUSION: FTY720 can inhibit S1P-induced corneal neovascularization.