Abstract:AIM: To study the effects of different concentrations of Coix seed oil on human retinal capillary endothelial cells(HRCECs)proliferation and vascular endothelial growth factor(VEGF)expression in high glucose environment.
METHODS: HRCECs extracted from human fresher eyeball and cultured in vitro, and ultimately used in the experiment were the growth of 3rd～4th cells, the experimental were divided into blank control group, low glucose control group, high glucose control group, high glucose +(50μL/mL, 100μL/mL, 200μL/mL)different concentrations Coix seed oil group. Detecting the multiplication of HRCECs by MTT, the immunocytochemical method was employed to detect the each group HRCECs of VEGF expression.
RESULTS:MTT assay results showed that: different concentrations of coix seed oil acted at HRCECs for 48h, inhibition of cell proliferation was significant difference compared with high glucose control group(P<0.05). Within 48h showed concentration dependence. There was no statistical difference between the low glucose group and high glucose control group(P>0.05). Immunocytochemical assay showed that: 50μL/mL, 100μL/mL, 200μL/mL Coix seed oil acted at HRCECs 48h, the expression of VEGF decreased significantly compared with the high glucose control group(P<0.05), and in a dose-dependent manner. However, in high glucose control group, the expression of VEGF was obvious higher than that of low glucose control group(P<0.05).
CONCLUSION:Coix seed oil can inhibit the HRCECs proliferation and suppress the VEGF expression in high glucose environment.
