Influence of iron ion on the formation of pseudomonas aeruginosa biofilm on the contact lens

Abstract:

AIM:To observe the influence of free iron, synthetic or biological iron chelating agent on the formation of pseudomonas aeruginosa biofilm on the contact lenses.

METHODS:Different concentration of free iron, different concentration and variety classes of synthesis iron and biological iron chelating agent were added into pseudomonas aeruginosa biofilm, and then the clump count on the biofilm was detected.

RESULTS:Low concentration of free iron increased the adhesive capacity of the bacterial, with 10μmol/L of iron had the most obvious effect. 0.1μmol/L of EDTA and lactoferrin significantly inhibited the bacterial count of pseudomonas aeruginosa biofilm.

CONCLUSION: Low concentration of free iron can promote the bacterial count of pseudomonas aeruginosa biofilm on the contact lens, the lack iron circumstance which formed by finite concentration lactoferrin and EDTA can prevent the formation of the biofilm.

Key words:pseudomonas aeruginosa; biofilm; contact lens; iron ion