Abstract:AIM: To investigate the distribution of adeno-associated-virus induced angiostatin Kringles5-GFP gene in ratsocular tissues after injected in vitreous cavity and to provide experiment basis to retinopathy treatment. METHODS: After injected rAAV-Kringle5-GFP with the titer 2.5×1012 vg/mL 10μL in vitreous cavity, the SD rats were sacrificed at day 1, 5, 10, 20, 40, 60 and 90. The distribution and expression of GFP in ocular tissues such as vitreous, retina, chloridum, iris and so on were observed under green microscopy. The expression degrees were recorded as -, +, ++, +++ and ++++. RESULTS: Kringle5 expressed in vitreous at the day 1 after injection and increased with the time. After 5 days, it expressed in retina, ranged from optic disc to peripheral retinal tissues. In iris and choroid, Kringle5 showed a strong expression. It could also be found in corneal endothelium and crystalline lens. CONCLUSION: The adeno-associated-virus induced angiostatin Kringles5-GFP gene can strongly express not only in retina but also in other ocular tissues. Thus, adeno-associated virus is the best vector for gene in curing retina disease.
