Cultivation and identification of nanobacteria in aqueous humor of patients with age-related cataract

doi:10.3969/j.issn.1672-5123.2012.01.31

Home > Archive>Volume 12, Issue 1, 2012 >98-100. DOI:10.3969/j.issn.1672-5123.2012.01.31

Cultivation and identification of nanobacteria in aqueous humor of patients with age-related cataract
DOI:
                        10.3969/j.issn.1672-5123.2012.01.31
                    
Author:
                        You-Ping ZhengYou-Ping Zheng
Department of Ophthalmology;
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Xing-Rui LanXing-Rui Lan
Department of Laboratory, Panyu District Hospital of Traditional Chinese Medicine, Guangzhou 511400, Guangdong Province,China；
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Shao-Qiong GuoShao-Qiong Guo
Department of Laboratory, Panyu District Hospital of Traditional Chinese Medicine, Guangzhou 511400, Guangdong Province,China；
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Jie-Mei LiuJie-Mei Liu
Department of Ophthalmology;
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Gui-Fang WangGui-Fang Wang
Department of Ophthalmology，the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province,China
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Fund Project:Panyu District Program for Tackling Key Problems in Science and Technology,Guangzhou,China（No.2009-Z-123-1）

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Abstract:

AIM:To isolate, culture and identify the nanobacteria (NB) from aqueous humor of patients with age-related cataract in our department. METHODS:Sample nanobacteria in aqueous humor of 30 patients with age-related cataract were collected for NB culture in this study. Immunohistochemistry, transmission electron microscopy (TEM) were used to investigate the appearance and components of cultured NB. RESULTS:Twelve aqueous samples out of 30 (40%) showed growth of NB, 1 of 5 showed growth of NB in the saline control group, but none growth in the control group. TEM showed needle-like nanobacteria aggregates of different sizes. CONCLUSION:For the first time we isolated, cultured and identified nanobacteria in aqueous of patients with age-related cataract, indicating that nanobacteria infection is related with age-related cataract.

Key words:age-related cataract; nanobacteria; infection; aqueous humor

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You-Ping Zheng, Xing-Rui Lan, Shao-Qiong Guo,/et al.Cultivation and identification of nanobacteria in aqueous humor of patients with age-related cataract. Guoji Yanke Zazhi( Int Eye Sci) 2012;12(1):98-100

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Received:November 16,2011
Revised:December 09,2011
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