Abstract:AIM:To study the effect of different media on the original rabbit lens epithelial cells·METHODS:Using the tissue block adhering wall method to culture the rabbit lens epithelial cells(RLEC) original generation in vitro,the situation of morphology,proliferation and differentiation of the original RLEC in the media containing different ingredients(DMEM low glucose,DMEM high glucose and DMEM/F12 medium) were learned·RESULTS:Cells cultured for 2 weeks on DMEM low and high glucose media began to differentiate and stop growing,the lens epithelial cells had significant fibrosis.DMEM/F12 medium made the cells grow well.When the cells passed on to the 5th generation,they began to transform into fibroblasts·CONCLUSION: The results indicates that the DMEM low glucose and DMEM high glucose media could inhibit the proliferation of the original RLEC to some extent,and DMED/F12 medium is suitable for the proliferation of original RLEC.
