Research on culture in vitro and proliferative activity of human Tenon’s capsule fibroblasts

Abstract:

AIM:To culture the human Tenon’s capsule fibroblast (HTF) in vitro and observe its proliferative activity.METHODS:Human Tenon’s capsule was obtained from strabismus surgery patients.Fibroblasts were primarily cultured and identified by immunofluorescence staining.Represented by growth curve,the cell viability was detected by cell counting kit-8(CCK-8).Cell cycle was observed by flow cytometer analysis and proliferation index were calculated.RESULTS:HTF were cultured successfully from culture in vitro.There was no significant difference of proliferative activity in different passages of HTF(P>0.05).CONCLUSION:HTF is an ideal target cell in the study on anti-fibrosis of human Tenon’s capsule due to its convenience of culturing and stable proliferative activity.

Key words:human Tenon’s capsule;fibroblast;cell culture;proliferation