Abstract:AIM:To establish the dry eye apoptosis model caused by the reduction of androgen levels,to explore the effect of Buddleja officinalis total flavonoids drug-containing plasma for activating the expression of AR mRNA;detect the expression of AR mRNA with the same method after using androgen receptor blockers,to determine relationship of the expression of AR mRNA with the binding effect of AR receptor in the Buddleja officinalis total flavonoids drug-containing plasma.METHODS:In vitro lacrimal gland epithelial cells were isolated and cultivated.The apoptosis of epithelial cells of rat lacrimal gland were inducted by H2O2,the dry eye apoptosis state caused by the reduction of androgen levels were established.There were the blank plasma group,Buddleia officinalis plasma total flavonoids interfere with drug-containing group,the intervention group of testosterone propionate,the expression of AR mRNA in each group was observed and androgen receptor blocker flutamide was used to explore the intended androgen effect of Buddleja officinalis total flavonoids.RESULTS:The results of Western blot showed that after the intervention of drug-containing plasma,the expression of AR mRNA in Buddleja officinalis drug-containing plasma total flavonoids intervention group and testosterone propionate intervention group were enhanced and the differences between the two groups were significant(P<0.01).After using the androgen receptor blocker in all groups,the expression of AR mRNA in each group had no difference(P>0.05).CONCLUSION:Buddleja officinalis total flavonoids drug-containing plasma can promote the expression of AR mRNA which is associated with the combination of Buddleja officinalis total flavonoids drug-containing plasma and the AR receptor,creating the same androgen effect with testosterone propionate.
