Abstract:AIM:To explore the microenvironment in which human bone marrow mesenchymal stem cells(BMSCs)differentiated into photoreceptor cells under nutrilits induction medium in vitroMETHODS:High-purity BMSCs could be obtained using lymphocyte separation medium by density gradient centrifugation.In the first stage the third passage cells were induced to neural precursor cells with medium including FGF,EGF and BDNF.The expression of the Nestin and MAP-2 were detected by immunocytochemistry.PEDF and taurine instead of FGF,EGF and BDNF in medium cultured the induced BMSCs furthermore 2 or 3 weeks when the positive expression rate of Nestin reached the highest level.Rhodopsin was researched during this second stage by immunocytochemistry and RT-PCR.RESULTS:Nestin could be detected from the third day after inducing differentiation,and reached its peak on the 12th day(90.9±2.6)%.MAP-2 also could be detected since the sixth day after inducing.Rhodopsin was detected during the farther inducing,and the positive rate was(20.7±3.8)% and(89.8±3.7)% in the second and third week respectively.CONCLUSION:BMSCs can differentiate into photoreceptor cells which express Rhodopsin under the condition by stages induction with FGF,EGF,BDNF,PEDF and taurine.
