Ting-Jun Fan
Key Laboratory for Corneal Tissue Engineering,Ocean University of China,Qingdao 266003,Shandong Province,ChinaJing Wang
Key Laboratory for Corneal Tissue Engineering,Ocean University of China,Qingdao 266003,Shandong Province,ChinaJun Zhao
Key Laboratory for Corneal Tissue Engineering,Ocean University of China,Qingdao 266003,Shandong Province,ChinaHong-Shou Yang
Key Laboratory for Corneal Tissue Engineering,Ocean University of China,Qingdao 266003,Shandong Province,ChinaWen-Zhuo Zhao
Key Laboratory for Corneal Tissue Engineering,Ocean University of China,Qingdao 266003,Shandong Province,ChinaXiu-Xia Yang
Key Laboratory for Corneal Tissue Engineering,Ocean University of China,Qingdao 266003,Shandong Province,ChinaR770.2
National High Technology Research and Development Program ("863" Program) of China (No. 2006AA 02A132)
·AIM:To investigate the inhibitory effect of transforming growth factor(TGF)-β2 on the proliferation of human corneal endothelial cells(HCEC)in vitro,and its cellular and molecular mechanisms.·METHODS:Synchronized HCEC,prepared from in vitro cultured HCEC line by serum-starvation,were treated with TGF-β2 at different concentration for different time duration,respectively.After HCEC treated with TGF-β2,the inhibitory effect of proliferation,phase status in cell cycle,and expression of p27kip1 and p21cip1 of HCEC was examined by methods of cell number counting,MTT staining,flow cytometry(FCM)and realtime PCR,respectively.·RESULTS:TGF-β2 at the concentration of 1-15μg/L had obvious inhibitory effects on HCEC proliferation in dose-and time-dependent manner,9μg/L was the peak concentration of TGF-β2 for inhibiting HCEC cell proliferation.After treated with 9μg/L TGF-β2,the population of HCEC in G1/G0 phase increased obviously in time-dependent manner.After HCEC treated with 9μg/L TGF-β2,the expression of p27kip1 increased obviously after 24 hours,and expression of p27kip1 and p21cip1 increased obviously 48 hours later in time-dependent manner.·CONCLUSION:TGF-β2 has obvious inhibitory effect on HCEC proliferation,and the inhibition is in dose-and time-dependent manner.The mechanism of TGF-β2 inhibition is most probably accomplished by inducing the in turn expression of p27kip1 and p21cip1 which keep HCEC rest on G1/G0 phase.·
Ting-Jun Fan, Jing Wang, Jun Zhao,/et al.Inhibitory effect of transforming growth factor-β2 on the proliferation of human corneal endothelial cells in vitro. Guoji Yanke Zazhi( Int Eye Sci) 2010;10(5):841-843
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