Effect of high levels of glucose on expressions of VEGF,EPO and EPOR in retinal Müller cells

Abstract:

·AIM:To observe the expressions of vascular endothelial growth factor(VEGF),erythropoietin(EPO),erythropoietin receptor(EPOR)mRNA in different levels of glucose in the retinal Müller cells of mice in vitro.·METHODS:Müller cells of new-born mice were cultured with enzyme digestion.Müller cells were separated and purified by blowing and striking method.The expressions of VEGF mRNA,EPO mRNA,and EPOR mRNA were detected by RT-PCR.·RESULTS:Retinal Müller cells were cultured successfully,90% of which were positively stained by glutamine synthetase(GS).The expressions of VEGF mRNA,EPO mRNA and EPOR mRNA in retinal Müller cells were significantly enhanced in the high glucose group which increased obviously(P<0.05),but the difference between 50mmol/L group and the 40mmol/L group did not have statistical significance or time dependence.·CONCLUSION:With glucose concentration elevating,VEGF,EPO,EPOR expression of cultured retinal Müller cells increased.·

Key words:retinal Müller cells;vascular endothelial growth factor;erythropoietin;erythropoietin receptor;neovascularization