Vascular endothelial growth factor-165b protects the blood-retinal barrier from damage after acute high intraocular pressure in rats
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Quan-Peng Zhang and Xi-Nan Yi. Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University, Haikou 571199, Hainan Province, China. 18889790750@163.com; yixn001@163.com

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Supported by the National Natural Science Foundation of China (No.81660217); Youth Foundation of the First Affiliated Hospital of Hainan Medical University (No.HYYFYPY201922).

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    Abstract:

    AIM: To elucidate the role of vascular endothelial growth factor-165b (VEGF-165b) in blood-retinal barrier (BRB) injury in the rat acute glaucoma model. METHODS: In this study, the rat acute high intraocular pressure (HIOP) model was established before and after intravitreous injection of anti-VEGF-165b antibody. The expression of VEGF-165b and zonula occludens-1 (ZO-1) in rat retina was detected by double immunofluorescence staining and Western blotting, and the breakdown of BRB was detected by Evans blue (EB) dye. RESULTS: The intact retina of rats expressed VEGF-165b and ZO-1 protein, which were mainly located in the retinal ganglion cell layer and the inner nuclear layer and were both co-expressed with vascular endothelial cell markers CD31. After acute HIOP, the expression of VEGF-165b was up-regulated; the expression of ZO-1 was down-regulated at 12h and then recovered at 3d; EB leakage increased, peaking at 12h. After intravitreous injection of anti-VEGF-165b antibody, the expression of VEGF-165b protein was no significantly changed; and the down-regulation of the expression of ZO-1 was more obvious; EB leakage became more serious, peaking at 3d. EB analysis also showed that EB leakage in the peripheral retina was greater than that in the central retina. CONCLUSION: The endogenous VEGF-165b protein may protect the BRB from acute HIOP by regulating the expression of ZO-1. The differential destruction of BRB after acute HIOP may be related to the selective loss of retinal ganglion cells.

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Jing Shen, Yi Li, Min Li, et al. Vascular endothelial growth factor-165b protects the blood-retinal barrier from damage after acute high intraocular pressure in rats. Int J Ophthalmol, 2022,15(8):1231-1239

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Publication History
  • Received:May 25,2021
  • Revised:May 27,2022
  • Adopted:
  • Online: July 28,2022
  • Published: