CCPG1 involved in corneal Aspergillus fumigatus infection
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Cheng-Ye Che. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, No.16 of Jiangsu Road, Shinan District, Qingdao 266000, Shandong Province, China. chechengye@126.com

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Supported by the National Natural Science Foundation of China (No.82171019); the Natural Science Foundation of Shandong Province (No.ZR2021MH368); Traditional Chinese Medicine Research Project of Qingdao (No.2020-zyy055); Shandong Qingdao Outstanding Health Professional Development Fund.

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    Abstract:

    AIM: To investigate whether non-canonical autophagy transport receptor cell cycle progression 1 (CCPG1) is involved in the corneal antifungal immune response. METHODS: Human corneal epithelial cells (HCECs) and human myeloid leukemia mononuclear cells (THP-1) macrophages stimulated by Aspergillus fumigatus (A. fumigatus) were used as cell models. The expression of CCPG1 mRNA was detected by qRT-PCR. Western blot was used to determine the protein expression of CCPG1 and interleukin-1β (IL-1β). The dectin-1 neutralizing antibody was used to detect the association between dectin-1 and CCPG1. Immunofluorescence was used to observe the colocalization of CCPG1 and C-type lectin-like receptor-1 (CLEC-1) in THP-1 macrophages. RESULTS: The expression of CCPG1 started to increase at 4h after infection and increased in a time-dependent manner in HCECs and THP-1 macrophages. With dectin-1 neutralizing antibody pretreatment, the expression of IL-1β was down-regulated. CCPG1 up-regulation in response to A. fumigatus infection was independent of dectin-1. Immunofluorescence showed the colocalization of CCPG1 and CLEC-1 in THP-1 macrophages. CONCLUSION: As a specific autophagy protein of non-canonical autophagy pathway, CCPG1 is involved in corneal infection with A. fumigatus.

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Li-Mei Wang, Xiao-Meng Chen, Hai-Jing Yan, et al. CCPG1 involved in corneal Aspergillus fumigatus infection. Int J Ophthalmol, 2022,15(4):541-546

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Publication History
  • Received:March 23,2021
  • Revised:December 08,2021
  • Adopted:
  • Online: March 29,2022
  • Published: