Protective effects of upregulated HO-1 gene against the apoptosis of human retinal pigment epithelial cells in vitro
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Yu Hong. Department of Ophthalmology, the Second Affiliated Hospital of Fujian Medical University. No.34 Zhongshan North Road, Quanzhou 362000, Fujian Province, China. hongyuccd@163.com

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Supported by Natural Science Foundation of Fujian Province (No.2017J01274); Health Science and Technology Program of Fujian Province (No.2015-ZQN-JC-26); Quanzhou Science and Technology Plan Project (No.2016Z053).

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    Abstract:

    AIM: To investigate the protective effect of heme oxygenase-1 (HO-1) against H2O2-induced apoptosis in human ARPE-19 cells. METHODS: The lentiviral vector expressing HO-1 was prepared and transfected into apoptotic ARPE-19 cells induced by H2O2. Functional experiments including cell counting kit-8 (CCK-8) assay, flow cytometry (FCM) and mitochondrial membrane potential assay were conducted. RESULTS: The ultrastructure of ARPE-19 cells was observed using transmission electron microscope (TEM). It was found that exogenous HO-1 significantly ameliorated H2O2-induced loss of cell viability, apoptosis and intracellular levels of reactive oxygen species (ROS) in ARPE-19 cells. The overexpression of HO-1 facilitated the transfer of nuclear factor erythroid-2-related factor 2 (Nrf2) from cytoplasm to nucleus, which in turn upregualted expressions HO-1 and B-cell lymphoma-2 (Bcl-2). Furthermore, HO-1 upregulation further inhibited H2O2-induced release of cysteinyl aspartate specific proteinase-3 (caspase-3). CONCLUSION: Exogenous HO-1 protect ARPE-19 cells against H2O2-induced oxidative stress by regulating the expressions of Nrf2, HO-1, Bcl-2, and caspase-3.

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Yu Hong, Yu-Ping Liang, Wei-Qi Chen, et al. Protective effects of upregulated HO-1 gene against the apoptosis of human retinal pigment epithelial cells in vitro. Int J Ophthalmol, 2021,14(5):649-655

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Publication History
  • Received:November 21,2020
  • Revised:March 25,2021
  • Adopted:
  • Online: March 29,2021
  • Published: