Role of microRNA-25 in high glucose cultured Müller glia
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Qing-Huai Liu and Song-Tao Yuan. Department of Ophthalmology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. liuqh@njmu.edu.cn; yuansongtao@vip.163.com

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Supported by National Key Research and Development Program of China (No.2017YFA0104101).

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    Abstract:

    AIM: To investigate the role of microRNA-25 (miR-25) in proliferation and apoptosis of retinal Müller glia (MG) under high glucose condition. METHODS: The purity of the cultured cells was verified by immunocytochemistry and flow cytometry using antibodies that specifically recognized MG. The expression level of miR-25 under normal and high glucose conditions were validated by quantitative reverse transcription polymerase chain reaction (RT-qPCR). miR-25 mimics and negative control were transfected into MG and multiple functional experiments including cell counting kit-8 assay, EDU assay, and flow cytometry were conducted to explore the effects of miR-25 on the proliferation and apoptosis of high glucose cultured MG (HGMG). RESULTS: Immunocytochemistry and flow cytometry confirmed the high purity of primary cultured MG. RT-PCR results showed that the expression level of miR-25 was significantly repressed in HGMG, while over-expression of miR-25 by miR-25 mimic markedly inhibited the high glucose induced cell apoptosis and promoted the proliferation of MG. CONCLUSION: The expression level of miR-25 is significantly downregulated in HGMG and its overexpression could attenuate the high glucose damages on MG by promoting proliferation and reducing apoptosis.

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Yu Liu, Han Shen, Song-Tao Yuan, et al. Role of microRNA-25 in high glucose cultured Müller glia. Int J Ophthalmol, 2021,14(5):643-648

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Publication History
  • Received:December 17,2020
  • Revised:February 18,2021
  • Adopted:
  • Online: March 29,2021
  • Published: