Anti-inflammatory effects of astaxanthin against fungal keratitis
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Gui-Qiu Zhao and Xu-Dong Peng. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, No.16 Jiangsu Road, Qingdao 266003, Shandong Province, China. zhaoguiqiu_good@126.com; doctorpxd@126.com

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Supported by the National Natural Science Foundation of China (No.81870632); Youth Project of Natural Science Foundation of Shandong Province (No.ZR2019BH004).

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    Abstract:

    AIM: To characterize effect of astaxanthin (ASX) in Aspergillus fumigatus (A. fumigatus) induced keratitis in mouse model. METHODS: In vivo, fungal keratitis mouse model was established in C57BL/6 mice using A. fumigatus, followed by ASX or dimethyl sulfoxide (DMSO) treatment. Clinical responses were evaluated by clinical score and myeloperoxidase (MPO) assay. Inflammatory cytokines were assessed by reverse-transcription polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, and enzyme-linked immuno sorbent assay (ELISA). RESULTS: In animal model, ASX improved corneal transparency and clinical response, suppressed the expression of inflammatory cytokine like IL-1β, TNF-α, and HMGB-1. Neutrophil levels have been shown to decrease in ASX-treated cornea by immunofluorescence and MPO. TLR2 and TLR4 levels were lower in ASX-treated group than DMSO-treated. CONCLUSION: ASX can suppress inflammatory response and reduce inflammatory cytokine production in mice model with A. fumigatus keratitis.

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Yu Huan, Xu-Dong Peng, Jing Lin, et al. Anti-inflammatory effects of astaxanthin against fungal keratitis. Int J Ophthalmol, 2020,13(11):1681-1688

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Publication History
  • Received:January 12,2020
  • Revised:August 14,2020
  • Adopted:
  • Online: September 17,2020
  • Published: