Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen
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Jin Ma. State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, Guangdong Province, China. zoc_majin@aliyun.com

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Supported by Natural Science Foundation of Guangdong Province (No.2016A030313364); Project of Administration of Traditional Chinese Medicine of Guangdong Province of China (No.20201065).

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    Abstract:

    AIM: To investigate the effects of collagen and opticin on the bioactivity of human retinal vascular endothelial cells (hRVECs), and explore its regulations by integrins and RhoA/ROCK1 signal pathway. METHODS: hRVECs were cultured in collagen and treated by opticin, and cell-based bioactivity assays of cell proliferation, migration, and adhesion were performed. The expression of integrin α2, integrin β1, RhoA and ROCK1 were examined with real-time PCR and Western blotting. RESULTS: Collagen could promote cell viability of proliferation and migration (all P<0.05), and enhance the mRNA expression of integrin α2, integrin β1, RhoA and ROCK1 (all P<0.05). Opticin could inhibit proliferation and migration ability of hRVECs cultured in collagen, and reduce the mRNA expression of integrin α2, integrin β1, RhoA and ROCK1 (all P<0.05). CONCLUSION: Collagen and opticin can affect bioactivity of hRVECs, which may be regulated by α2-, β1-integrins and RhoA/ROCK1 signal pathway.

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Yue Xing, Xue-Ke Li, Si-Duo Lu, et al. Regulation of opticin on bioactivity of retinal vascular endothelial cells cultured in collagen. Int J Ophthalmol, 2020,13(8):1202-1209

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Publication History
  • Received:March 15,2020
  • Revised:April 29,2020
  • Adopted:
  • Online: June 22,2020
  • Published: