Cytotoxic effect of specific T cells from mice with experimental autoimmune uveitis on murine photoreceptor cells
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Hong-Sheng Bi. Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250002, Shandong Province, China. hongshengbi1@163.com

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Supported by the National Natural Science Foundation of China (No.81873163); the Natural Science Foundation of Shandong Province (No.ZR2017LH042); the Development Project of Medicine and Health Science Technology of Shandong Province (No.2015BJYB28; No.2017WS073); the Development Project of Science and Technology of Traditional Chinese Medicine of Shandong Province (No.2015-145); the Excellent Youth Science Foundation of Shandong University of Traditional Chinese Medicine (No.2018zk26).

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    Abstract:

    AIM: To investigate the cytotoxic effect of specific T cells from mice with experimental autoimmune uveitis (EAU) as well as their secreted interferon (IFN)-γ and interleukin (IL)-17A on murine photoreceptor (661W) cells. METHODS: An EAU model was established in female mice by injection of interphotoreceptor retinoid binding protein (IRBP) emulsion supplemented with complete Freund’s adjuvant (CFA) and Mycobacterium tuberculosis (TB). On day 12 after induction of EAU, specific T cells from spleen and lymph node tissues were isolated and cultured for 4d and the levels of IFN-γ and IL-17A in the supernatants were determined by enzyme-linked immunosorbent assays (ELISAs). T cells and their supernatants were added to 661W cells to observe the alteration of cell morphology; IFN-γ and IL-17A were separately added to 661W cells to observe the effect of IFN-γ and IL-17A on cell proliferation. RESULTS: The levels of IFN-γ and IL-17A in the T cell supernatants were 1568.64±38.79 pg/mL and 1456.57±46.98 pg/mL, respectively. The supernatants apparently inhibited 661W cell proliferation (P<0.05). T cells could also attach to the surface of 661W cells, and IFN-γ showed a more serious cytotoxic effect on 661W cells than IL-17A, inhibiting cell proliferation (P<0.01). CONCLUSION: IFN-γ and IL-17A from T cells of EAU mice model can exert cytotoxic effects on murine photoreceptor cell proliferation, and IFN-γ shows more serious cytotoxic effects on murine photoreceptor cells than IL-17A.

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Zheng-Feng Liu, Feng Zhang, Da-Dong Guo, et al. Cytotoxic effect of specific T cells from mice with experimental autoimmune uveitis on murine photoreceptor cells. Int J Ophthalmol, 2020,13(8):1180-1186

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Publication History
  • Received:March 31,2019
  • Revised:March 10,2020
  • Adopted:
  • Online: June 22,2020
  • Published: