Blockade of insulin receptor substrate-1 inhibits biological behavior of choroidal endothelial cells
Author:
Corresponding Author:

Pei-Rong Lu and Gao-Qin Liu. Department of Ophthalmology, the First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou 215006, Jiangsu Province, China. lupeirong@suda.edu.cn; liugaoqin2006@sina.com

Affiliation:

Clc Number:

Fund Project:

Supported by the National Natural Science Foundation in China (No. 81671641; No.81970830; No.31600736); Suzhou Municipal Natural Science Foundation (No.SYS201745); Soochow University Doctoral Academic Talents Program (No.5832001313); Jiangsu Provincial Medical Youth Talent (No.QNRC2016718); Jiangsu Provincial Medical Innovation Team (No.CXTDA2017039); Jiangsu Provincial Natural Science Foundation (No.BK20151208); the Soochow Scholar Project of Soochow University (No.R5122001).

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM: To investigate the effects of blockade of insulin receptor substrate-1 (IRS-1) on the bio-function of tube formation of human choroidal endothelial cells (HCECs). METHODS: Quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were performed to determine the expression level of IRS-1 and phospho-IRS-1 in HCECs. Tube formation of HCECs was analyzed using three dimensional in vitro Matrigel assay with or without IRS-1 blockage via IRS-1 inhibitor (GS-101) and vascular endothelial growth factor receptor 2 (VEGFR2) inhibitor. In addition, cell counting kit (CCK)-8 and Transwell migration assay were exerted to analyze the effects of blockade of IRS-1 on the bio-function of proliferation and migration of HCECs, respectively. The apoptosis of HCECs was examined using flow cytometry (FCM). RESULTS: RT-PCR and Western blot revealed that IRS-1 phospho-IRS-1 were expressed in HCECs and the expression level was enhanced by stimulation of VEGF-A. The number of tube formation was decreased significantly in GS-101 treated groups compared to phosphate buffered saline (PBS) treated control groups. Furthermore, both cell proliferation and migration of HCECs were decreased in the presence of GS-101. FCM analysis showed that the apoptosis of HCECs was enhanced when the cells were treated with GS-101. Western blot also showed that the expression level of cleaved-caspase 3 in GS-101 treated group was higher than that in control group. CONCLUSION: Blockade of IRS-1 can inhibit tube formation of HCECs through reducing cell proliferation and migration and promoting cell apoptosis.

    Reference
    Related
    Cited by
Get Citation

Yi-Yong Qian, Hong-Ya Wu, Gao-Qin Liu, et al. Blockade of insulin receptor substrate-1 inhibits biological behavior of choroidal endothelial cells. Int J Ophthalmol, 2019,12(9):1386-1394

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:July 27,2018
  • Revised:December 15,2018
  • Adopted:
  • Online: August 02,2019
  • Published: