Broccoli sprouts juice prevents lens protein aggregation in streptozotocin-induced diabetic rat
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Nina Handayani. Department of Ophthalmology, Faculty of Medicine, Universitas Brawijaya, Dr. Saiful Anwar General Hospital, Malang 65145, East Java, Indonesia. ninahdyn@gmail.com

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    Abstract:

    AIM: To investigate the effect of broccoli sprouts juice in preventing lens protein aggregation in diabetic rat model. METHODS: Totally 25 male Wistar rats were divided into negative control group, diabetic group without juice treatment as positive control and diabetic group given broccoli sprouts juice 0.25, 0.5 and 1 g/d for 28d. Diabetic rat model was obtained by administering a single dose streptozotocin 65 mg/kg intraperitoneal. At the end of the study, all rats were examined for fasting blood sugar level (FBS), visual cataract score using slit lamp biomicroscope and lens αB-crystallin expression using Western blot method. Statistical analysis was performed using one way ANOVA with post hoc test, correlation test, and simple linear regression. RESULTS: Positive control group had the highest cataract score and lens aggregated αB-crystallin expression. Broccoli sprout juice dose of 1 g/d group had the mildest cataract score, as well as the expression of lens aggregated αB-crystallin compared to treatments groups 1 and 2, opposite to lens native αB-crystallin expression. The broccoli sprout juice groups gave a significant decrease in cataract score, and also in lens aggregated αB-crystallin expression in diabetic rat models (P<0.05). CONCLUSION: Broccoli sprout juice has a significant effect in preventing lens protein aggregation in diabetic rat model. The higher dose gives better visual cataract scores, lower lens aggregated αB-crystallin expression and higher lens native αB-crystallin expression.

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Ayu Puspitasari, Nina Handayani. Broccoli sprouts juice prevents lens protein aggregation in streptozotocin-induced diabetic rat. Int J Ophthalmol, 2019,12(9):1380-1385

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Publication History
  • Received:November 30,2018
  • Revised:March 12,2019
  • Adopted:
  • Online: August 02,2019
  • Published: