Metabolic profile analysis of free amino acids in experimental autoimmune uveoretinitis rat plasma
Author:
Corresponding Author:

Jing-Zhen Tian. No.4655, Daxue Road, Jinan 250355, Shandong Province, China. tianjingzhen@163.com; Hong-Sheng Bi. No.48, Yingxiongshan Road, Jinan 250002, Shandong Province, China. hongshengbi1@163.com

Affiliation:

Clc Number:

Fund Project:

Supported by the National Natural Science Foundation of China (No.81373826; No.81674032); Natural Science Foundation of Shandong Province (No.ZR2014HQ074); Key Development & Research Program of Shandong Province (No.2017GSF19110).

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM: To determine the differences of amino acid (AA) levels in experimental autoimmune uveoretinitis (EAU). METHODS: AA analysis of the plasma samples in EAU rats induced by interphotoreceptor retinoid-binding protein emulsion were performed with high performance liquid chromatography (HPLC) and phenylisothiocyanate (PITC) pre-column derivation methods were performed. Using partial least squares discriminant analysis (PLS-DA), the potential biomarkers were identified in EAU rat plasma, and the metabolic pathways related to EAU were further analyzed. RESULTS: The method results showed that linear (r≥0.9957), intra-day reproducible [relative standard deviation (RSD)=0.04%-1.33%], inter-day reproducible (RSD=0.06%-2.07%), repeatability (RSD=0.03%-0.89%), stability (RSD=0.05%-2.48%) and recovery (RSD=1.98%-4.39%), with detection limits of 0.853-11.4 ng/mL. The metabolic profile in EAU rats was different from that in the control groups five AAs concentrations were increased and nine AAs were reduced. Moreover, five metabolic pathways were related to the development of EAU. CONCLUSION: The developed method is a simple, rapid and convenient for determination of AAs in EAU rat plasma, and these findings will provide a comprehensive insight on the metabolic profiling of the pathological changes in EAU.

    Reference
    Related
    Cited by
Get Citation

Jun-Guo Guo, Xin-Miao Guo, Xing-Rong Wang, et al. Metabolic profile analysis of free amino acids in experimental autoimmune uveoretinitis rat plasma. Int J Ophthalmol, 2019,12(1):16-24

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:January 17,2018
  • Revised:November 21,2018
  • Adopted:
  • Online: January 03,2019
  • Published: