Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide
Author:
Corresponding Author:

Yi-Qiao Xing. Eye Center, Renmin Hospital of Wuhan University, Jiefang Road 238#, Wuhan 430060, Hubei Province, China. xingyiqiao1111@163.com

Affiliation:

Clc Number:

Fund Project:

Supported by Ph.D. Programs Foundation of Ministry of Education of China (No.20130141120052).

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM: To explore the effect of ciliary neurotrophic factor (CNTF) on retinal ganglion cell (RGC)-5 induced by hydrogen peroxide (H2O2). METHODS: After cell adherence, RGC-5 culture medium was changed to contain different concentrations of H2O2 from 50 to 150 μmol/L at four time points (0.5, 1, 1.5 and 2h) to select the concentration and time point for H2O2 induced model. Two different ways of interventions for injured RGC-5 cells respectively were CNTF as an addition in the culture medium or recombinant lentiviral plasmid carrying CNTF gene transfecting bone mesenchymal stem cells (BMSCs) for co-culture with RGC-5. RESULTS: Compared to the control group, H2O2 led to RGC-5 death closely associated with concentrations and action time of H2O2 and we chose 125 μmol/L and 2h to establish the H2O2-induced model. While CNTF inhibited the loss of RGC-5 cells obviously with a dose-dependent survival rate. Nevertheless two administration routes had different survival rate yet higher rate in recombinant lentiviral plasmid group but there were no statistically significant differences. CONCLUSION: Both the two administration routes of CNTF have effects on RGC-5 cells induced by H2O2. If their own advantages were combined, there may be a better administration route.

    Reference
    Related
    Cited by
Get Citation

Wen-Jun Wang, Wei Jin, An-Huai Yang, et al. Protective effects of ciliary neurotrophic factor on the retinal ganglion cells by injure of hydrogen peroxide. Int J Ophthalmol, 2018,11(6):923-928

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:July 18,2017
  • Revised:November 22,2017
  • Adopted:
  • Online: June 12,2018
  • Published: