Suppression of fibrosis in human pterygium fibroblasts by butyrate and phenylbutyrate
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Noriaki Maeshige. Division of Nutrition and Metabolism, Department of Biophysics, and Department of Rehabilitation Science, Graduate School of Health Sciences, Kobe University, Tomogaoka 7-10-2, Suma-ku, Kobe, Japan. nmaeshige@pearl.kobe-u.ac.jp

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Supported by JSPS KAKENHI (No.23592648).

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    Abstract:

    AIM: To evaluate the antifibrogenic effects of butyrate or phenylbutyrate, a chemical derivative of butyrate, in human pterygium fibroblasts. METHODS: Human pterygium fibroblasts obtained from patient pterygium tissue were treated with butyrate or phenylbutyrate for 48h. Expression of α-smooth muscle actin, collagen I, collagen III and matrix metalloproteinase-1 mRNA was measured by quantitative real-time reverse transcription polymerase chain reaction, and acetylated histone was evaluated by Western blotting. RESULTS: Butyrate inhibited α-smooth muscle actin, type III collagen and matrix metalloproteinase-1 expressions, and phenylbutyrate inhibited types I and III collagen and matrix metalloproteinase-1 expressions without changing cell viability as well as both of these increased histone acetylation. These results suggested that butyrate and phenylbutyrate suppress fibrosis through a mechanism involving histone deacetylase inhibitor. CONCLUSION: This indicates that butyrate or phenylbutyrate have antifibrogenic effects in human pterygium fibroblasts and could be novel types of prophylactic and/or therapeutic drugs for pterygium, especially phenylbutyrate, which does not have the unpleasant smell associated with butyrate.

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Yuka Koga, Noriaki Maeshige, Hiroto Tabuchi, et al. Suppression of fibrosis in human pterygium fibroblasts by butyrate and phenylbutyrate. Int J Ophthalmol, 2017,10(9):1337-1343

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Publication History
  • Received:February 18,2017
  • Revised:April 25,2017
  • Adopted:
  • Online: September 05,2017
  • Published: