Expression of lens-related microRNAs in transparent infant lenses and congenital cataract
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Supported by the Natural Science Foundation of China (No.81470614); the Fundamental Research Funds for the Central Universities sponsored by Xi’an Jiaotong University (No.xjj2013067); Youth Foundation of the First Affiliated Hospital, Medical College, Xi’an Jiaotong University (No.2014YK7); Scientific Research Funds for the Health and Family Planning of Shaanxi Province (No.2016D068).

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    Abstract:

    AIM: To identify the expression of lens-related microRNAs (miRNAs) in the central epithelium of transparent infant lenses and congenital cataract. METHODS: Lens-related miRNAs were retrieved from PubMed database. The expression levels of these miRNAs in transparent infant lenses and congenital cataract were determined by stem-loop reverse transcription-polymerase chain reaction (RT-PCR). miRanda algorithm was used to predict the target genes of these differentially expressed miRNAs. The target mRNA was validated. RESULTS: Six lens-related miRNAs were retrieved from screening PubMed database. The most abundant miRNA in transparent infant lenses according to stem-loop RT-PCR was miR-184. miR-182 was up-regulated in congenital cataract. Contrarily, miR-204 and miR-124 was down-regulated. miR-204 exhibited a more significant decrease in expression than miR-124. In addition, Meis2 was predicted to be the target of miR-204 using miRanda algorithm. miR-204 mimic/antagomir transfection experiments suggested the negative correlation between the expression of miR-204 and Meis2. CONCLUSION: The expression levels of miR-182, miR-204 and miR-124 differ between the central epithelium of transparent infant lens and congenital cataract, suggesting their involvement in the pathogenesis of congenital cataract. miR-204 may act via silencing Meis2 to regulate lens development and congenital cataract formation.

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Chang-Rui Wu, Min Ye, Li Qin, et al. Expression of lens-related microRNAs in transparent infant lenses and congenital cataract. Int J Ophthalmol, 2017,10(3):361-365

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Publication History
  • Received:December 05,2016
  • Revised:February 06,2017
  • Adopted:
  • Online: March 14,2017
  • Published: