Effects of retinoic acid receptor-γ on the Aspergillus fumigatus induced innate immunity response in human corneal epithelial cells
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Gui-Qiu Zhao. Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China. zhaoguiqiu_good@126.com

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Supported by National Natural Science Foundation of China (No.81470609; No.81500695; No.81300730); Key Project of Natural Science Foundation of Shandong Province (No.ZR2012HZ001); Youth Natural Science Foundation of Shandong Province (No.ZR2013HQ007).

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    Abstract:

    AIM: To explore the effects of retinoic acid receptor-γ (RARγ) on innate immune responses against Aspergillus fumigatus (A. fumigatus) in cultured human corneal epithelial cells (HCECs). METHODS: The HCECs were stimulated with A. fumigatus hyphae for 0, 2, 4, 8, 12 and 16h. RARγ mRNA and protein levels were tested by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Then HCECs were pretreated with or without BMS961 (RARγ agonist, 1 μg/mL). The mRNA and protein expression of Dectin-1 and the downstream cytokines (TNF-α and IL-6) were determined by qRT-PCR, Western blot and enzyme-linked immunosorbent assay (ELISA). RESULTS: The expression of RARγ was upregulated after stimulation with A. fumigatus. RARγ mRNA began to rise at 4h and peaked at 8h (P<0.001). The protein of RARγ reached to the peak at 16h (P<0.001). Pretreated with BMS961 before A. fumigatus hyphae stimulation, expression of Dectin-1, TNF-α and IL-6 decreased dramatically at mRNA and protein levels. CONCLUSION: HCECs can express RARγ and A. fumigatus hyphae infection can increase RARγ expression. BMS961 can inhibit the expression of Dectin-1 and pro-inflammatory cytokines, and play an anti-inflammatory role in innate immune responses against A. fumigatus.

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Xiao-Chen Wang, Gui-Qiu Zhao, Jing Lin, et al. Effects of retinoic acid receptor-γ on the Aspergillus fumigatus induced innate immunity response in human corneal epithelial cells. Int J Ophthalmol, 2016,9(12):1713-1718

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Publication History
  • Received:October 12,2015
  • Revised:July 28,2016
  • Adopted:
  • Online: December 07,2016
  • Published: