Immune oppression array elucidating immune escape and survival mechanisms in uveal melanoma
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Wen-Bin Wei. Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Intraocular Tumor Diagnosis and Treatment, Beijing Ophthalmology and Visual Science Key Lab, No.1 Dong Jiao Min Xiang, Dong Cheng District, Beijing 100730, China. weiwenbintr@163.com

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Supported by National Natural Science Foundation of China (No.81570891; No.81272981); the Beijing Municipal Administration of Hospitals’ Ascent Plan (No.DFL20150201); Science & Technology Project of Beijing Municipal Science & Technology Commission (No.Z151100001615052); Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (No.ZYLX201307); Beijing Natural Science Foundation (No.7151003); Advanced Health Care Professionals Development Project of Beijing Municipal Health Bureau (No.2014-2-003).

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    Abstract:

    AIM: To examine the genetic profile of primary uveal melanoma (UM) as compared to UM in immune escape. METHODS: Dendritic cells (DC) loaded with lysates of UM cells of high metastatic potential were used to stimulate CTLs(CTLs). When CTLs co-cultured with the UM cells, most UM cells could be eliminated. Survival UM cells grew slowly and were considered to be survival variants and examined by a microarray analysis. These differential genes were analyzed further with Venn Diagrams and functions related to immune escape. We additionally examined transcriptional changes of manually selected survival variants of UM cells and of clinical UM samples by quantitative real-time polymerase chain reaction (qRT-PCR), and analyzed the correlation of these expressions and patients’ survival. RESULTS: Gene expression analyses revealed a marked up-regulation of SLAMF7 and CCL22 and a significant down-regulation of KRT10, FXYD3 and ABCC2. The expression of these genes in the relapsed UM was significantly greater than those in primary UM. UM patients with overexpression of these genes had a shorter survival period as compared with those of their underexpression. CONCLUSION: Gene expression, in particular of SLAMF7, CCL22, KRT10, FXYD3 and ABCC2, differed between primary UM cells and survival variants of UM cells.

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Fang Hou, Qi-Ming Huang, Dan-Ning Hu, et al. Immune oppression array elucidating immune escape and survival mechanisms in uveal melanoma. Int J Ophthalmol, 2016,9(12):1701-1712

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Publication History
  • Received:August 29,2016
  • Revised:September 27,2016
  • Adopted:
  • Online: December 07,2016
  • Published: