Effects of dexamethasone and HA1077 on actin cytoskeleton and β-catenin in cultured human trabecular meshwork cells
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Bo Gong; Chun-Tao Lei. Sichuan Provincial Key Laboratory for Disease Gene Study, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu 610072, Sichuan Province, China. gongbo2007@hotmail.com; hblct@163.com

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Supported by the Natural Science Foundation of China (No.81300768; No.81371048; No.81670853); Science and Technology Department of Sichuan Province of China (No.2015HH0031); Health and Family Planning Commission of Sichuan Province of China (No.100539; No. 090505; No.090514).

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    Abstract:

    AIM: To investigate the effects of dexamethasone (DEX) and 1-(5-isoquinolinesulfonyl)-homopiperazine (HA1077) on actin cytoskeleton and β-catenin in cultured human trabecular meshwork (HTM) cells. METHODS: The HTM cells were separated from human eyeball and cultured in vitro. They were divided into control group, DEX (1×10-6 mol/L) group, HA1077 (3×10-5 mol/L) group, and DEX (1×10-6 mol/L) and HA1077 (3×10-5 mol/L) group. Actin cytoskeleton and β-catenin in HTM cells of the four groups were examined by immunofluorescence and Western blot analyses. RESULTS: In DEX group, there were reorganization of actin cytoskeleton and formation of cross linked actin networks (CLANs), which were partially reversed in DEX and HA1077 group. DEX treatment also induced an increased expression of β-catenin, which was obviously reduced in DEX and HA1077 group. Meanwhile, the cultured HTM cells in HA1077 group had lower expression of β-catenin than that in the control group. CONCLUSION: Our results show that HA1077 can reverse the changes of actin organization and expression of β-catenin induced by DEX in cultured HTM cells, suggesting that HA1077 may play an important role in increasing outflow and reducing intraocular pressure.

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Jie Peng, Xiao-Yun Feng, Zi-Meng Ye, et al. Effects of dexamethasone and HA1077 on actin cytoskeleton and β-catenin in cultured human trabecular meshwork cells. Int J Ophthalmol, 2016,9(10):1376-1380

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Publication History
  • Received:February 22,2016
  • Revised:May 16,2016
  • Adopted:
  • Online: October 13,2016
  • Published: