The role of Dectin-1/Raf-1 signal cascade in innate immune of human corneal epithelial cells against Aspergillus fumigatus infection
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Gui-Qiu Zhao. Department of Ophthalmology, the Affiliated Hospital of Qingdao University, 16th Jiangsu Road, Qingdao 266003, Shandong Province, China. zhao_guiqiu@126.com

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Supported by National Natural Science Foundation of China (No.81170825; No.81470609; No.81500695); Specialized Research Fund for the Doctoral Program of Higher Education (No.20123706110003); the Youth Natural Science Foundation of Shandong Province (No.ZR2013HQ007); the Key Project of Natural Science Foundation of Shandong Province (No.ZR2012HZ001).

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    Abstract:

    AIM: To investigate the expression of the v-raf-1 murine leukemia viral oncogene homolog 1 (Raf-1) and its role in the innate immune response of human corneal epithelial cells (HCECs) infected by Aspergillus fumigatus. METHODS: HCECs were cultured in vitro. They were randomly divided into 4 groups, including control group, Aspergillus fumigatus group, GW5074 (an inhibitor of Raf-1) group and Laminarin [an inhibitor of Dendriti-cell-associated C-type lectin 1 (Dectin-1)] group. The protein expression level of total Raf-1 and p-Raf-1was measured by Western blot. The expression of IL-6 and IL-8 mRNA in each group was detected by real-time polymerase chain reaction. RESULTS: In Aspergillus fumigatus group, total Raf-1 protein levels in HCECs remained unchanged at 5, 15, 30 and 45min after infection, while p-Raf-1 expression was significantly enhanced at 30min after infection compared with control group. However, the expression of p-Raf-1 was apparently declined after treated with GW5074 or Laminarin compared with Aspergillus fumigatus group. The expression levels of IL-6, IL-8 mRNA were significantly increased after stimulation with fumigatus compared with control group. Pre-treated with GW5074 significantly inhibited Aspergillus fumigatus-induced upregulation of IL-8 and IL-6. CONCLUSION: Aspergillus fumigatus stimulation can elevate the expression of p-Raf-1 in HCECs in vitro. Dectin-1/Raf-1 signal pathway may play a role on regulating the expression of inflammatory cytokines, including IL-6 and IL-8.

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Gui-Qiu Zhao, Jing Lin, Li-Ting Hu, et al. The role of Dectin-1/Raf-1 signal cascade in innate immune of human corneal epithelial cells against Aspergillus fumigatus infection. Int J Ophthalmol, 2016,9(10):1371-1375

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Publication History
  • Received:February 24,2016
  • Revised:April 12,2016
  • Adopted:
  • Online: October 13,2016
  • Published: