Construction of adenovirus vectors encoding the lumican gene by gateway recombinant cloning technology
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Jing-Xiang Zhong. Department of Ophthalmology, the First Affiliated Hospital of Jinan University, 613 Huangpu Road, Guangzhou 510630, Guangdong Province, China. lafangme@126.com

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Supported by the Natural Science Foundation of Guangdong Province (No.2015A030310158; No.2014A030313359); the Fundamental Research Funds for the Central Universities (No.21611446); the Scientific and Cultivation Foundation of the First Affiliated Hospital of Jinan University (No.2015201).

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    Abstract:

    AIM: To construct adenovirus vectors of lumican gene by gateway recombinant cloning technology to further understand the role of lumican gene in myopia. METHODS: Gateway recombinant cloning technology was used to construct adenovirus vectors. The wild-type (wt) and mutant (mut) forms of the lumican gene were synthesized and amplified by polymerase chain reaction (PCR). The lumican cDNA fragments were purified and ligated into the adenovirus shuttle vector pDown-multiple cloning site (MCS)-/internal ribozyme entry site (IRES)/enhanced green fluorescent protein (EGFP). Then the desired DNA fragments were integrated into the destination vector pAV.Des1d yielding the final expression constructs pAV.Ex1d-cytomegalovirus (CMV)>wt-lumican/IRES/EGFP and pAV.Ex1d-CMV>mut-lumican/IRES /EGFP, respectively. RESULTS: The adenovirus plasmids pAV.Ex1d-CMV>wt-lumican/IRES/EGFP and pAV.Ex1d-CMV>mut-lumican/IRES/EGFP were successfully constructed by gateway recombinant cloning technology. Positive clones identified by PCR and sequencing were selected and packaged into recombinant adenovirus in HEK293 cells. CONCLUSION: We construct adenovirus vectors containing the lumican gene by gateway recombinant cloning technology, which provides a basis for investigating the role of lumican gene in the pathogenesis of high myopia.

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Gui-Fang Wang, Bing Qi, Lei-Lei Tu, et al. Construction of adenovirus vectors encoding the lumican gene by gateway recombinant cloning technology. Int J Ophthalmol, 2016,9(9):1271-1275

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Publication History
  • Received:September 08,2015
  • Revised:February 14,2016
  • Adopted:
  • Online: September 12,2016
  • Published: