Expression of microRNAs in fibroblast of pterygium
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Ungsoo Samuel Kim. Department of Ophthalmology, Kim’s Eye Hospital, Youngdeungpo 4th 156, Youngdeungpo-gu, Seoul 07301, Korea. ungsookim@kimeye.com

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Supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology Grant 2010-0009023; Konyang University Myunggok Research Fund of 2013.

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    Abstract:

    AIM: To screen microRNAs (miRNAs) and set up target miRNAs in pterygium. METHODS: Primary fibroblasts were isolated from pterygium and Tenon's capsule and cultured. Immunocytochemical analysis and Western blotting were performed to confirm the culture of fibroblasts. In all, 1733 miRNAs were screened in the first step by using GeneChip® miRNA3.0 Array. Specific miRNAs involved in the pathogenesis of pterygium were subsequently determined using the following criteria: 1) high reproducibility in a repetitive test; 2) base log value of >7.0 for both control and pterygial fibroblasts; and 3) log ratio of >1.0 between pterygial fibroblasts and control fibroblasts. RESULTS: Primary screening showed that 887/1733 miRNAs were up-regulated and 846/1733 miRNAs were down-regulated in pterygial fibroblasts compared with those in control fibroblasts. Of the 1733 miRNAs screened, 4 miRNAs, namely, miRNA-143a-3p, miRNA-181a-2-3p, miRNA-377-5p and miRNA-411a-5p, met the above-mentioned criteria. Primary screening showed that these 4 miRNAs were up-regulated in pterygial fibroblasts compared with control fibroblasts and that miRNA-143a-3p had the highest mean ratio compared with the miRNAs in control fibroblasts. CONCLUSION: miRNA-143a-3p, miRNA-181a-2-3p, miRNA-377-5p and miRNA-411a-5p are up-regulated in pterygial fibroblasts compared with control fibroblasts, suggesting their involvement in the pathogenesis of pterygium.

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Joon H. Lee, Sun-Ah Jung, Young-A Kwon, et al. Expression of microRNAs in fibroblast of pterygium. Int J Ophthalmol, 2016,9(7):967-972

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Publication History
  • Received:September 02,2015
  • Revised:January 19,2016
  • Adopted:
  • Online: July 12,2016
  • Published: