Endothelial nitric oxide synthase deficiency influences normal cell cycle progression and apoptosis in trabecular meshwork cells
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Chan Li. Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, 1 youyi Road, Yuzhong District, Chongqing 400016, China. chanlichch@163.com; Hong Yang. Department of Ophthalmology, Southwest Hospital, Third Military Medical University, No.30 Gaotanyan, Shapingba District, Chongqing 400038, China. hongyangdff@163.com

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Supported by Science Fund for Youths (No. 81300763).

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    Abstract:

    AIM: To clarify how the endothelial nitric oxide synthase (eNOS, NOS3) make effect on outflow facility through the trabecular meshwork (TM). METHODS: Inhibition of NOS3 gene expression in human TM cells were conducted by three siRNAs. Then the mRNA and protein levels of NOS3 in siRNA-treated and negative control (NC) cells were determined, still were the collagen, type IV, alpha 1 (COL4A1) and fibronectin 1 by real-time PCR and Western blot analysis. In addition, NOS3 concentrations in culture supernatant fluids of TM cells were measured. Cell cycle and cell apoptosis analysis were performed using flow cytometry. RESULTS: The mRNA level of NOS3 was decreased by three different siRNA interference, similar results were obtained not only of the relative levels of NOS3 protein, but also the expression levels of COL4A1 and fibronectin 1. The number of cells in S phase was decreased, while contrary result was obtained in G2 phase. The number of apoptotic cells in siRNA-treated groups were significant increased compared to the NC samples. CONCLUSION: Abnormal NOS3 expression can make effects on the proteins levels of extracellular matrix component (e.g. fibronectin 1 and COL4A1). Reduced NOS3 restrains the TM cell cycle progression at the G2/M-phase transition and induced cell apoptosis.

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Qiong Liao, Yan-Ming Huang, Wei Fan, et al. Endothelial nitric oxide synthase deficiency influences normal cell cycle progression and apoptosis in trabecular meshwork cells. Int J Ophthalmol, 2016,9(6):799-803

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Publication History
  • Received:August 26,2015
  • Revised:October 27,2015
  • Adopted:
  • Online: June 16,2016
  • Published: