Expression of transcription factors Slug in the lens epithelial cells undergoing epithelial-mesenchymal transition induced by connective tissue growth factor
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1Medical School of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China;
2Department of Ophthalmology, the First Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China

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Supported by National Natural Science Foundation of China (No.81470614, No.81460163, No.81300786); Fundamental Research Funds for the Central Universities (No.xjj2014146); Specialized Research Fund for the Doctoral Program of Higher Education (No.20133601120012); Key International Communication Project of Shaanxi province (No.2012KW-31).

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    Abstract:

    AIM:To investigate the expression of transcription factors Slug in human lens epithelial cells (HLECs) undergoing epithelial-mesenchymal transition (EMT) induced by connective tissue growth factor (CTGF).METHODS: HLECs were treated with CTGF of different concentrations (20, 50 and 100 ng/mL) or without CTGF (control) for 24h. The morphological changes of HLECs were analysed by microscopy. The expression and cellular localization of Slug was evaluated by immumo-fluorescence. Expressions of Slug, E-cadherin and alpha smooth muscle actin (α-SMA) were further determined by Western blot analysis. RESULTS: HLECs showed spidle fibrolasts-like characteristics and loosely connected each other after CTGF treatment. The immuno-fluorescence staining indicated that Slug was localized in the nuclei and its expression was induced by CTGF. The relative expressions of Slug protein were 1.64±0.11, 1.96 ±0.03, 3.12 ±0.10, and 4.08±0.14, respectively, in response to control group and treatment with CTGF of 20, 50 and 100 ng/mL (F=443.86, P<0.01). The increased Slug protein levels were correlated well with up-expression of α-SMA (0.78±0.05, 0.85±0.06, 2.17±0.15, 2.86±0.10; F=449.85, P<0.01) and down-expression of E-cadherin (2.50±0.11, 1.79±0.26, 1.05±0.14, 0.63±0.08; F=101.55, P<0.01). CONCLUSION: Transcription factor Slug may be involved in EMT of HLECs induced by CTGF in vitro.

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Ying-Na Wang, Li Qin, Jing-Ming Li, et al. Expression of transcription factors Slug in the lens epithelial cells undergoing epithelial-mesenchymal transition induced by connective tissue growth factor. Int J Ophthalmol, 2015,8(5):872-876

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Publication History
  • Received:December 25,2014
  • Revised:April 24,2015
  • Adopted:April 24,2015
  • Online: October 09,2015
  • Published: