Efficacy of the nucleotide-binding oligomerzation domain 1 inhibitor Nodinhibit-1 on corneal alkali burns in rats
Author:
Corresponding Author:

Affiliation:

1Affiliated Eye Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China;
2Fujian Provincial Key Laboratory of Ophthalmology and Visual Science, Eye Institute of Xiamen University, Xiamen 361102, Fujian Province, China;
3Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China

Clc Number:

Fund Project:

Supported by the National Natural Science Foundation of China (No.81400372, No.81300729, No.81160118); Clinical Medicine Research Special-purpose Foundation of China (No.L2012052); Jiangxi Province Sailing Engineering (No.2014022); ShanHai Foundation of China (No.2013SH008); Science Technology Foundation of Jiangxi Province (No.20151BBG70223); Youth Science Foundation of Jiangxi Province (No.20151BAB215016); Natural Science Foundation of Fujian Province (No.2015J05170); Education Department Youth Scientific Research Foundation (No.GJJ14170); Health Development Planning Commission Science Foundation of Jiangxi Province (No.20155154).

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM:To evaluate the therapeutic effect of Nodinhibit-1 on alkali-burn-induced corneal neovascularization (CNV) and inflammation.The nucleotide-binding oligomerzation domain 1 (NOD1) is a potent angiogenic gene.METHODS:The alkali-burned rat corneas (32 right eyes) were treated with eye drops containing Nodinhibit-1 or phosphate buffered solution (PBS, PH 7.4) only, four times per day. CNV and inflammation were monitored using slit lamp microscopy, and the area of CNV was measured by formula. Vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) was determined by Western blot analysis. The TUNEL assay was used to assess the corneal apoptosis cells.RESULTS:Alkali-burn-induced progressive CNV and inflammation in the cornea. After treatment for 7d and 14d, there were statistically significant differences in the CNV areas and inflammatory index on that between two group(P<0.05, respectively). Epithelial defect quantification showed a significant difference between the two groups at days 4 and 7 after the alkali burns (P<0.05). The apoptotic cells on days 1, 4, and 7 between the two groups showed significant differences at all time points (P<0.05, respectively). Compared to that in control group, the protein level of VEGF expression was significantly reduced whereas the PEDF expression was increase in the Nodinhibit-1 groups on day 14 (P<0.05, respectively=.CONCLUSION:Topical application of 10.0 μg/mL Nodinhibit-1 may have potential effect for the alkali burn-induced CNV and inflammation. The effect of Nodinhibit-1 on CNV may be by regulation the equilibrium of VEGF and PEDF in the wounded cornea.

    Reference
    Related
    Cited by
Get Citation

Xu Huang, Yun Han, Yi Shao, et al. Efficacy of the nucleotide-binding oligomerzation domain 1 inhibitor Nodinhibit-1 on corneal alkali burns in rats. Int J Ophthalmol, 2015,8(5):860-865

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:June 11,2014
  • Revised:March 10,2015
  • Adopted:March 10,2015
  • Online: October 09,2015
  • Published: