Suppression of laser-induced choroidal neovascularization by intravitreal injection of tristetraprolin
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Supported by Biomedical Research Institute Fund (GNUHBRIF-2013-0002) from the Gyeongsang National University Hospital.
Conflicts of Interest: Cho YW, None; Han YS, None; Chung IY, None; Kim SJ, None; Seo SW, None; Yoo JM, None; Park JM, None. ACKNOWLEDGEMENTS This work was genuinely contributed by Yong Seop Han, and data and conclusions were endorsed by Yong Wun Cho.

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    Abstract:

    AIM:To examine the effect of intravitreal adenoviral vector-mediated tristetraprolin (Ad-TTP) on VEGF mRNA expression in a rat model of laser-induced choroidal neovascularization.METHODS:Ad-TTP was prepared using a commercial kit. Retinal laser-induced photocoagulation (10 spots per eye) was performed on rats in this experimental choroidal neovascularization (CNV) model. Rats were divided into four groups:control (single intravitreal injection of balanced salt solution, n=10), laser-induced CNV (photocoagulation only, n=20), laser-induced CNV plus Ad-TTP injection (photocoagulation plus a single intravitreal Ad-TTP injection, n=20) and Ad-TTP injection only (n=10). Changes in choroidal morphology were evaluated in ten rats in the laser only and the laser plus Ad-TTP groups. Two weeks after laser injury, the size of CNV was calculated by perfusion with high-molecular-weight fluorescein isothiocyanate (FITC)-dextran. VEGF mRNA expression in retina-choroid tissue from ten rats in each group was measured by reverse transcription polymerase chain reaction (RT-PCR).RESULTS:Two weeks after treatment, the area of laser-induced CNV was reduced by approximately 60% in the rats given the Ad-TTP injection compared with that in the laser-only group. There was a tendency toward decreased VEGF mRNA expression in the Ad-TTP injection groups.CONCLUSION:A single intravitreal injection of Ad-TTP significantly suppressed CNV size in this experimental laser-induced CNV model. Ad-TTP injection also decreased VEGF mRNA expression compared with that in the laser-induced CNV group. The present study is meaningful as the first study to investigate the effect of tristetraprolin delivered via intravitreal injection.

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Yong Wun Cho, Yong Seop Han, In Young Chung, et al. Suppression of laser-induced choroidal neovascularization by intravitreal injection of tristetraprolin. Int J Ophthalmol, 2014,7(6):952-958

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Publication History
  • Received:October 28,2013
  • Revised:August 25,2014
  • Adopted:August 25,2014
  • Online: December 15,2014
  • Published: