Mitochondrial proteomic analysis of ecdysterone protection against oxidative damage in human lens epithelial cells
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Supported by Fujian Province Health Department Fund (No.2009-1-30); Fujian Province Department of Education Issues (No.JA10176)
Conflicts of Interest: Feng CY, None; Huang XR, None; Qi MX, None; Tang SW, None; Chen S, None; Hu YH, None; Ke FJ, None; Wang X, None.

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    Abstract:

    AIM:To investigate the protective effects of the natural medicinal monomer ecdysterone (ECR) with estrogenic activity against oxidative damage in human lens epithelial cells B3 (HLE-B3) caused by hydrogen peroxide 21(H2O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects.METHODS: HLE-B3 cells were treated with H2O2 (300μmol/L), β-estuarial (E2; 10-8mol/L) and H2O2, ECR (10-6mol/L) and H2O2, or left untreated. Altered expression of all mitochondrial proteins was analyzed by protein array and surface-enhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). The mass/charge (M/Z) ratios of each peak were tested by the Kruskal-Wallis rank sum test, and the protein peak value of the M/Z ratio for each treatment by pair comparison was analyzed with the Nemenyi test.RESULTS: H2O2 up-regulated expression of two protein spots (with M/Z of 6 532 and 6 809). When E2 mitigated the oxidative damage, the expression of one protein spot (M/Z 6 532) was down-regulated. In contrast, ECR down-regulated both of protein spots (M/Z 6 532 and 6 809).CONCLUSION: ECR could effectively inhibite H2O2 induced oxidative damage in HLE-B3 cells. The protein spot at M/Z of 6 532 might be the target spot of ECR against oxidative damage induced by H2O2.

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Chun-Yan Feng, Xiu-Rong Huang, Ming-Xin Qi, et al. Mitochondrial proteomic analysis of ecdysterone protection against oxidative damage in human lens epithelial cells. Int J Ophthalmol, 2014,7(1):38-43

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Publication History
  • Received:May 02,2012
  • Revised:October 12,2012
  • Adopted:October 12,2012
  • Online: February 20,2014
  • Published: