Expression of dectin-1 during fungus infection in human corneal epithelial cells
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Foundation: Supported by National Natural Science Foundation of China (No.81170825; No.81300730)
Conflicts of Interest: Li C, None; Zhao GQ, None; Che CY, None; Li N, None; Lin J, None; Xu Q, None; Wang Q, None; Liu Y, None; Qiu S, None.

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    Abstract:

    AIM:To evaluate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) in human corneal epithelial (HCE) cells infected by fungus.METHODS:A total of 20 cases of healthy donor corneas were group A, and 20 patients (20 eyes) suffered from fungal keratitis (FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus (AF) antigens in vitro. The expression of dectin-1 mRNA was measured by real-time qPCR at the stimulation of 0, 4, 8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately.RESULTS: Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin-1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h, and dectin-1 protein expression increased after stimulation at 24h.CONCLUSION: Dectin-1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin-1 in HCE cells in vitro.

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Cui Li, Gui-Qiu Zhao, Cheng-Ye Che, et al. Expression of dectin-1 during fungus infection in human corneal epithelial cells. Int J Ophthalmol, 2014,7(1):34-37

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Publication History
  • Received:September 22,2012
  • Revised:October 21,2013
  • Adopted:October 21,2013
  • Online: February 20,2014
  • Published: