Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells
Author:
Corresponding Author:

Affiliation:

Clc Number:

Fund Project:

Foundation of Heilongjiang Educational Committee Project, China (No. 12511313); Heilongjiang Provincial Foundation for Youths Project, China (No. QC2011C119); Research Program of Heilongjiang Provincial Health Office, China (No. 2009-197)

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM:To explore the potential mechanism of molecular hydrogen in the regulation of miRNA expression and signal-modulating activities.METHODS:Retinal microglia cells were activated by Lipopolysaccharides (LPS) and then treated with hydrogen-saturated medium or normal medium without hydrogen. qRT-PCR was used to detect the expression difference in miR-9, miR-21 and miR-199 between these two groups. Moreover, the expression of LPS-induced signaling proteins, including Myd88, IKK-β, NF-κB, and PDCD4, were detected by Western blotting.RESULTS:The results demonstrated a marked down-regulation of miR-9 and miR-21 and up-regulation of miR-199 by hydrogen treatment; the expression of Myd88 and IKK-β was decreased after hydrogen treatment, whereas PDCD4 was increased, and there was no significant change in NF-κB expression.CONCLUSION:The results in the present study indicate that miR-9, miR-199 and miR-21 play an important role in the anti-inflammatory regulation of LPS-activated microglia cells by molecular hydrogen, which will help to explain the protective mechanism of molecular hydrogen against inflammatory injury.

    Reference
    Related
    Cited by
Get Citation

Guo-Dan Liu, Hong Zhang, Lin Wang, et al. Molecular hydrogen regulates the expression of miR-9, miR-21 and miR-199 in LPS-activated retinal microglia cells. Int J Ophthalmol, 2013,6(3):280-285

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:September 03,2012
  • Revised:April 20,2013
  • Adopted:April 20,2013
  • Online: June 24,2013
  • Published: