Effect of brain-derived neurotrophic factor on c-jun expression in the rd mouse retina
Author:
Corresponding Author:

Affiliation:

Clc Number:

Fund Project:

National Natural Science Foundation of China (No. 30973262)Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100730, China

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    AIM: To determine the location of c-jun protein, dynamic changes in c-jun mRNA and protein expression, and ultrastructure characteristics in the rd mouse retina, following a single dose of brain-derived neurotrophic factor (BDNF) in a short period of time. METHODS: A single intravitreal injection of BDNF at two dosages (25μg/L or 50μg/L) was given to the right eye of the rd mouse at age 2 and 3 weeks respectively. Two weeks after injection, the location of c-jun protein in the retina was observed by immunofluorescence detection, c-jun mRNA and protein expression in retinas were detected by quantitative real time polymerase chain reaction (RT-PCR) and western immunoblotting analysis, ultrastructure characteristics of retinas were detected by transmission electron microscope (TEM) observation. RESULTS: c-jun protein was expressed in the inner nuclear layer (INL) of retina. BDNF at two dosages (25μg/L and 50μg/L) increased c-jun mRNA expression at PN-4 weeks respectively (P1=0.019, P2= 0.021). 50μg/L BDNF increased c-jun protein expression at PN-4 weeks (P =0.000). The retinal ultrastructure was improved. CONCLUSION: The effects of BDNF exerts on the c-jun expression in the retina are dose-dependent and time-dependent, which may mediate photoreceptor rescue indirectly in the pathological process of retinitis pigmentosa (RP) at early stage.

    Reference
    Related
    Cited by
Get Citation

Rui Chen, Xiao-Bei Yin, Chun-Xia Peng, et al. Effect of brain-derived neurotrophic factor on c-jun expression in the rd mouse retina. Int J Ophthalmol, 2012,5(3):266-271

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
Publication History
  • Received:April 10,2012
  • Revised:May 25,2012
  • Adopted:
  • Online:
  • Published: