Side population cells from HXO-Rb44 retinoblastoma cell line have cancer-initiating property
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Supported by National Natural Science Foundation of China (No.81072108; No:31050110429); China Postdoctoral Science Foundation(No.20090450167; No.201003676); PH.D Programs Foundation of Ministry of Education of China(No.20090201120068); Scientific and Technological Cooperation Projects of Shaanxi Province, China(2010K14-02); The Fundamental Research Funds for the Central Universities

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    Abstract:

    AIM: To ascertain whether side population(SP) cells in HXO-Rb44 retinoblastoma cell line have cancer stem cell-like property in vitro and in vivo . METHODS: We analyzed and sorted SP from HXO-Rb44 retinoblastoma cell line by Hoechst 33342 staining on flow cytometry. SP and NSP cells were determined their ability of proliferation and self-renewal by SP reanalysis, soft agar assay and tumor sphere assay in vitro. Clone formation was detected by seeding HXO-Rb44 and HXO-Rb44 -RFP cells into soft agar. The expression of ABCG2, MDRI, Bmi-1 and Oct-4 was determined by RT-PCR between SP and non-SP (NSP) cells. Moreover, they were injected into nude mice to determine their tumorigency in vivo. RESULTS: SP from HXO-Rb44 retinoblastoma cell line could grow clonally in soft agar assays and form tumor spheres from single cells in conditioned media. The expressions of ABCG2, MDRI, Bmi-1 and Oct-4 were significantly higher in SP than NSP cells. As few as SP cells resulted in tumor formation in 6 of 12 injected sites, however, the injection of NSP cells failed to form new tumor. CONCLUSION: SP cells isolated by Hoechst 33342 from the HXO-Rb44 retinoblastoma cell line had property of high tumorigency in vivo and in vitro. Therefore, SP might be a target while developing retinoblastoma therapies.

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Jun-Jun She, Peng-Ge Zhang, Xiang-Ming Che, et al. Side population cells from HXO-Rb44 retinoblastoma cell line have cancer-initiating property. Int J Ophthalmol, 2011,4(5):461-465

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