Abstract:To explore the dynamic expression and correlation among telomerase catalytic subunit (TERT), proliferating cell nuclear antigen（PCNA）and antiapoptosis protein Bcl-2 which relate to cell proliferation in epiretinal membrane （ERM）of rat traumatic proliferative vitreoretinopathy (PVR). · METHODS: S-P technique was applied for immunohisto- chemical staining of ERM of traumatic PVR with TERT, PCNA and Bcl-2 antibody. HE staining was also carried out. The staining results were analyzed with image analysis system. · RESULTS: The positive rate and average A of PCNA protein were up regulated at first and then down regulated, with the peak value in 14 days Group, which was significantly different from those in 7 days Group and 28 days Group. The positive rate and average A of TERT and Bcl-2 were also up regulated at first and then down regulated, with the peak value in 14 days Group and 21 days Group, which were significantly different from those in 7 days Group. There was significant correlation among PCNA, Bcl-2 and TERT protein expression (P ≤0.01). · CONCLUSION: TERT and Bcl-2 take part in the regulation of proliferative cells in ERM of traumatic PVR, with high correlation with the dynamic changes of cell proliferation.
