目的：评估声乐训练对干眼人群泪膜稳定性的即时效应。

方法：前瞻性交叉对照研究。于2026年4月1日至2026年4月20日招募自愿参与者\〖经眼表疾病指数-6(OSDI-6)量表评分为4-8分的轻度至中度干眼者\〗。随机分为两组进行不同顺序的两次干预，A组第一次干预中进行50 min标准化声乐训练，B组为空白对照； 第二次干预两组交换干预方式，干预之间的洗脱期为1 wk。记录每次干预后即时的干眼症状变化问卷(CDES-Q)评分、每次干预前后的非侵入性泪膜破裂时间(NIBUT)，包括首次NIBUT和平均NIBUT以及泪河高度(TMH)。

结果：本研究共招募符合条件的参与者32例64眼，所有参与者均按计划完成了此次研究。随机分为两组进行不同顺序的两次干预，A组16例32眼中男3例，女13例，平均年龄61.69±4.56岁，平均OSDI-6量表评分5.750±1.183分； B组16例32眼中男3例，女13例，平均年龄63.19±1.56岁，平均OSDI-6量表评分5.250±1.438分。两组参与者干预前一般资料比较均无差异(P>0.05)。声乐训练干预后，首次NIBUT净效应量为1.883 s(P=0.042)，平均NIBUT净效应量为2.076 s(P=0.019)，TMH无明显变化(P=0.652)，CDES-Q量表评分差值为-47.188(P<0.001)。进一步个体数据分析显示59%的参与者在声乐训练后首次NIBUT延长，50%的参与者平均NIBUT延长。

结论：声乐训练可即时改善泪膜稳定性，该疗法用于干眼治疗，为后续规模化与长期研究提供了参考依据。

METHODS:A prospective crossover controlled study. From April 1 to April 20, 2026, volunteers with mild-to-moderate dry eye \〖Ocular Surface Disease Index-6(OSDI-6)score: 4-8\〗 were enrolled. Participants were randomly allocated into two groups to receive two interventions in different sequences. Group A underwent a 50-min standardized vocal training session during the first intervention, while Group B served as a blank control; The two groups crossed over to the alternative intervention for the second session, with a 1-week washout period between the two interventions. The immediate post-intervention changes in Dry Eye Symptoms Questionnaire(CDES-Q)score, pre-and post-intervention non-invasive tear film breakup time(NIBUT), including first NIBUT and average NIBUT, as well as tear meniscus height(TMH), were recorded for each session.

RESULTS:A total of 32 eligible participants(64 eyes)were enrolled and completed the study per protocol. The participants were randomly assigned to two groups with different intervention sequences. Group A(16 participants, 32 eyes)comprised 3 males and 13 females, with a mean age of 61.69±4.56 y and a mean OSDI-6 score of 5.750±1.183. Group B(16 participants, 32 eyes)comprised 3 males and 13 females, with a mean age of 63.19±1.56 y and a mean OSDI-6 score of 5.250±1.438.There were no significant differences in baseline characteristics between the two groups(P>0.05). After vocal training intervention, the net effect size for first NIBUT was 1.883 s(P=0.042), and for average NIBUT was 2.076 s(P=0.019). No significant change was observed in TMH(P=0.652). The CDES-Q score difference was -47.188(P<0.001). Further individual data analysis revealed that 59% of participants exhibited prolonged first NIBUT and 50% exhibited prolonged average NIBUT after vocal training.

CONCLUSION: Vocal training can immediately improve tear film stability. This therapeutic approach shows promise for dry eye management and provides a reference for future large scale and long-term investigations.