[关键词]
[摘要]
N-糖基化是一种重要的蛋白质翻译后修饰，可以影响蛋白质的功能，在多种生物学过程中起着关键作用。目前的研究已经发现了蛋白质N-糖基化的改变与多种眼部疾病的发生发展密切相关。而N-糖基化检测方法能够发现蛋白中N-糖链的改变，因此，了解各类检测方法及其在眼部疾病中的应用价值具有重要意义。文章系统综述了蛋白质N-糖基化检测方法的研究现状，包括毛细管电泳、液相色谱及质谱检测等技术，并详细讨论了这些技术在糖尿病视网膜病变、年龄相关性黄斑变性、春季角结膜炎和特应性角结膜炎等眼部疾病研究中的具体应用。
[Key word]
[Abstract]
N-glycosylation is a crucial posttranslational modification of proteins that can modulate their functions and plays key roles in various biological processes. Current research has revealed that alterations in protein N-glycosylation are closely associated with the pathogenesis and progression of multiple ocular diseases. N-glycosylation detection methods can identify changes in N-glycans on proteins; therefore, understanding these techniques and their application value in ocular diseases is important. This article provides a systematic review of the current state of N-glycosylation detection methods, including techniques such as capillary electrophoresis, liquid chromatography, and mass spectrometry. It also offers a detailed discussion on the specific applications of these technologies in the study of various ocular diseases, including diabetic retinopathy, age-related macular degeneration and vernal keratoconjunctivitis/atopic keratoconjunctivitis.
[中图分类号]
[基金项目]
广东省普通高校重点科研平台和项目(No.2022LSYS006,2023KCXTD013)
